The 32-year-old -- who is out of favour at Arsenal and was stripped of the captaincy last month -- and Mario Lemina both tested positive last week and missed Gabon's opening 1-0 win over Comoros on Monday.
However, coach Patrice Neveu is isolated after testing positive according to the Gabonese football federation.
Gabon have had a troubled build-up to the biennial continental showpiece tournament.
The Gabonese government had to resolve a dispute between the players and the federation over match bonuses while the Confederation of African Football (CAF) scolded them for breaking the rules in changing hotel in Yaounde.
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