Sevilla emerged victorious, securing a record-extending seventh title after a tense penalty shootout against AS Roma.
The match ended 1-1 after extra time, with Sevilla prevailing 4-1 on penalties.
Sevilla's goalkeeper, Yassine Bounou, played a pivotal role in their triumph, becoming the hero of the shootout.
Bounou made two crucial saves, denying Roma's Gianluca Mancini and Roger Ibanez, while his teammates converted all four of their penalty kicks.
Bounou also pipped the man of the match award from intended Chris Smalling with his heroics at the penalty having the final say on the night.
The match had started with Paulo Dybala giving Roma the lead with a well-taken goal in the 35th minute.
However, Sevilla responded strongly in the second half, equalizing through an unfortunate own goal by Roma's Gianluca Mancini.
The contest remained tightly contested throughout extra time, with both teams pushing for a winner.
Roma's Chris Smalling came agonizingly close to sealing the victory for the Italians with a looping header from a corner, only to see the ball rebound off the crossbar.
With the score deadlocked at the end of extra time, the match headed into a nerve-wracking penalty shootout.
Sevilla held their nerve, converting all their penalties with precision.
It was Gonzalo Montiel, who had previously converted the winning penalty for Argentina in the last year's World Cup final, who once again demonstrated his composure by netting the decisive spot-kick for Sevilla.
The penalty had to be retaken due to encroachment, but Montiel made no mistake with his second attempt.
The defeat proved to be a bitter blow for Roma's coach, Jose Mourinho, who experienced his first loss in a major European final in six attempts.