She suffered the injury in her opening match in Madrid earlier this month, before struggling in a second-round loss to Sara Sorribes Tormo.
“Unfortunately I’m going to have to withdraw from Rome as the injury which I picked up last week in Madrid hasn’t healed yet,” the Japanese star said in a statement.
“It’s an Achilles injury so I need to be careful, especially in advance of Roland Garros.”
The former world number one has never reached the final of a clay-court tournament, but will be hoping to put that record straight at the French Open which starts on May 22.
Osaka, currently ranked 38th, has shown signs of a return to form this year.
The 24-year-old reached the Miami Open final, where she lost to world number one Iga Swiatek, in April.
She has been replaced in the Rome draw by Spain’s Nuria Parrizas Dias.
Oladimeji Balogun is a graduate of the University of Ilorin where he studied Microbiology, he is a w...
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